CUSTOM ITEM: HPLC Column, Bidentate C8, 4um, 3.0mm ID x 50mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑05P‑3 Cogent Bidentate C8™ HPLC Column is a custom‑manufactured, mid‑bore analytical column designed to deliver alternative reversed‑phase selectivity with reduced solvent consumption compared to standard 4.6 mm ID formats. Built on Cogent TYPE‑C™ silica hydride technology, this column provides excellent selectivity, faster equilibration, and extremely robust column lifetime under demanding analytical conditions.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than conventional C8 columns, yet less hydrophobic than Bidentate C18™, making it well suited when C18 retention is excessive but stronger retention than standard C8 is desired. Unlike traditional silica‑based C8 phases, this column can also retain select hydrophilic compounds, offering increased method flexibility.
The 3.0 mm internal diameter enables lower flow rates and significantly reduced mobile‑phase usage, while the 50 mm length supports short run times comparable to 4.6 mm ID columns. Importantly, these solvent‑saving benefits are achieved without requiring specialized low‑flow instrumentation, making this format easy to integrate into standard analytical HPLC systems.
The column is also stable at very low pH (below pH 2) with no damage, expanding the usable method window beyond that of conventional C8 columns.
Why Choose the 3.0 × 50 mm Bidentate C8™ Format
This configuration is commonly selected when:
C18 columns provide excessive retention
Standard C8 columns do not provide enough hydrophobic interaction
Solvent‑use reduction is a priority without changing instrumentation
Fast run times are required with balanced reversed‑phase selectivity
The mid‑bore 3.0 mm ID geometry provides an effective compromise between throughput and efficiency, while TYPE‑C™ silica hydride chemistry ensures robustness and reproducibility.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase surface chemistry
Reduced silanol activity and secondary interactions
Faster equilibration than conventional silica‑based C8 columns
Extremely robust column lifetime
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention
Alternative selectivity when C18 is overly retentive
Solvent‑Efficient Analytical Geometry
3.0 mm ID × 50 mm length
Short run times similar to 4.6 mm ID columns
Lower flow rates reduce solvent consumption
No specialized low‑flow equipment required
Broad Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Suitable for aggressive analytical methods
Typical Applications
Routine analytical HPLC
Situations where C18 retention is excessive
Alternative reversed‑phase selectivity
High‑throughput analytical workflows
Low‑pH and aqueous‑rich methods
Solvent‑reduction initiatives
Custom Order Information
This product is classified as a Custom Item. Custom items are manufactured to order, non‑returnable, and cannot be canceled once production has begun.
Please contact Customer Service for lead time confirmation, documentation, or customization inquiries.